Permission to conduct this study was given by the institutional review board of the National Institute of Preventive and Social Medicine (NIPSOM), Bangladesh (Ref NIPSOM/IRB/2017/09). The Shaheed Suhrawardy Medical College Hospital and Dhaka Dental College Hospital provided the necessary documentation. Both written and verbal consent were taken before initiating the interview. Participants received an overview of the study’s goals, and those who consented were eventually included. No compensation was given to the participants, and data anonymity was strictly maintained.

This cross-sectional study was conducted from January 1 to December 31, 2022, in two tertiary-level hospitals in Dhaka South named Shaheed Suhrawardy Medical College Hospital and Dhaka Dental College Hospital in Dhaka City. Mothers of children aged 5-9 years who visited these tertiary hospitals were interviewed through a semistructured questionnaire.

To observe the overall scenario including questionnaire information, possible sampling techniques, and approximate nonresponse rate in the study, we first performed a pretest of the study. The pretesting was conducted among 50 mothers of children aged 5‐9 years in the Sapporo Dental College & Hospital located at Dhaka North.

A convenience sampling technique was followed for this study. During the literature search, no study was found that assessed the knowledge and practices toward children’s oral hygiene among Bangladeshi mothers. However, a study was found from India with a similar sociodemography. Mohandass et al [20] showed that the prevalence of adequate knowledge and practices were 58% and 57%, respectively. The sample size for this study was calculated using the below equation.

The sample size when P=.58 for the mother’s knowledge was:

Similarly, the sample size when P=.57 for the mother’s practice level was:

Therefore, we initially chose a maximum of 377 as the required sample size. Considering a maximum 5% nonresponse rate (based on pretesting), we rounded up this figure and selected 400 as an approximate sample size for the study.

The inclusion criteria for this study were mothers of Bangladeshi nationality who were living in Dhaka for at least 1 year, mothers of children aged 5‐9 years, and mothers who provided consent and agreed to participate in the study. The exclusion criteria for the study were mothers who were not Bangladeshi but currently living in Dhaka, mothers of children older than 10 years or younger than 5 years, and mothers younger than 21 years or older than 48 years.

Respondents’ sociodemographic variables such as age (21-48 years), religion (Muslim, non-Muslim), educational status (up to primary, secondary, higher secondary, and bachelor’s degree or higher), occupational status (housewife, working), family type (nuclear, joint), family size (<5 persons, ≥5 persons), and monthly family income (≤20,000 BDT, 20,001‐40,000 BDT, ≥40,001 BDT; a currency exchange rate of 101.85 BDT=US $1 was used) were the independent variables in this study.

The study used 15 variables to assess mothers’ knowledge and 13 to assess their children’s practices related to oral hygiene (Multimedia Appendices 2 and 3). Both knowledge and practice questions were adopted from the existing literature and revised according to our selection criteria. The summation scoring technique was used in computation, and the descriptive statistics, including percentiles, were observed. The range for the knowledge and practice scores were 1-15 and 1-13, respectively. According to the percentile approach, knowledge was classified into four levels: poor (<25% percentile cut point: ≤9.999), moderately average (25%‐49% percentile cut point: 10.0‐11.99), average (50%‐74% percentile cut point: 12.0‐12.99), and good knowledge (≥75% percentile cut point: ≥13.0) [24]. Practices were also classified into four levels: poor (<25% percentile cut point:≤4.99), moderately average (25%‐49% percentile cut point: 5.0‐5.999), average (50%‐74% percentile cut point: 6.0‐6.99), and good practices (≥75% percentile cut point: ≥7.0). For all cases, the cut points were statistically evident [25,26].

To ensure the reliability and validity of the study findings, we observed the reliability analysis for both knowledge and practice variables, yielding a Cronbach α; the reliability coefficient values for the variables related to knowledge and practice were found to be 0.78 and 0.81, indicating acceptable internal consistency.

Descriptive statistics were performed to present participants’ sociodemographic characteristics and mean knowledge and practice scores. The Pearson χ² test and Pearson correlation coefficient were used as a bivariate analysis. Since both knowledge and practice scores did not follow normality, we performed the Mann-Whitney U test and Kruskal-Wallis 1-way ANOVA test to show the mean knowledge and practice score variations between two (eg, housewife vs working mother) and more than two groups (eg, different age groups), respectively. Necessary assumptions were checked before performing the statistical analysis. All the data management and statistical analyses were carried out through SPSS Statistics 27.0 (IBM Corp). The P value was observed for all the cases at a 5% level, and 95% was considered as the CI [27-29].

The majority of the respondents (n=209, 52.3%) were within the 21‐30 years age group, followed by 44% (n=176) in the 31‐40 years age group. Most (n=57, 39.3%) respondents had a secondary level of education. Most were Muslims (n=388, 97%) and housewives (n=347, 86.8%). Many of the respondents (n=157, 39.3%) had a monthly family income of 20,001‐40,000 BDT (US $206.19-$392.73) per month. About 13.3% (n=53) of mothers were working (Table 1).

Multimedia Appendix 4 shows the mothers’ knowledge scores regarding their children’s oral hygiene. Among the 400 mothers, more than 90% (n=360) knew the importance of brushing teeth, while 82.3% (n=329) and 80.8% (n=323) knew the recommended frequency and appropriate time for brushing teeth, respectively. Surprisingly, only 29.5% (n=118) and 38.5% (n=154) knew the duration for brushing teeth and that fluoride protects against caries, respectively. However, most of the respondents knew about the “importance of cleaning tongue” (n=365, 91.3%), “gingival disease common cause of gum bleeding” (n=286, 71.5%), “brushing and flossing protect against bleeding gum” (n=243, 60.8%), “yellow coating plaque” (n=362, 90.5%), “sugary item cause caries” (n=387, 96.8%), “soft drinks cause caries” (n=295, 73.8%), and “regular brushing protects against caries” (n=380, 95%).

Multimedia Appendix 5 shows the individual distribution of mothers’ practices regarding their children’s oral hygiene. Most (n=381, 95.3%) of the mothers reported that their child brushed their teeth regularly, 99% (n=396) of children used a toothbrush, 62% (n=248) changed their toothbrush every 3‐4 months or if the bristles were frayed, 97.8% (n=391) used their toothpaste, and 77.8% (n=311) rinsed their mouth after eating. Surprisingly, 44.3% (n=177) of children brushed their teeth twice daily, 42% (n=168) cleaned their tongues, and 2.8% (n=11) used floss. Only 12.5% (n=50) were given sugary items with meals, and 0.3% (n=1) were taken to dentists every 6 months.

Figure 1 depicts the level of knowledge and practices of mothers regarding their children’s oral hygiene and the association with the mother’s educational status. Only 41.3% (n=165) had good knowledge, while 18.5% (n=74) had poor knowledge (Figure 1A). Similarly, only 45.5% (n=182) of the mothers showed good practices, while 16.2% (n=65) showed poor practice levels (Figure 1B).

A total of 66.7% (10/15) of mothers aged 41-48 years had good knowledge regarding their children’s oral hygiene. The Pearson χ² association test revealed that mothers’ knowledge levels were significantly associated with age (P=.01), education (P<.001), family size (P=.03), and monthly income (P<.001; Table 2).

Table 3 represents the association between mothers’ sociodemographic characteristics and their practices regarding their children’s oral hygiene. The analysis found that more than half (n=8, 53.3%) of older-aged mothers had good practices, and 66.7% (n=60) of mothers with a bachelor’s degree or higher showed good practices regarding their children’s oral hygiene. The educational status (P=.002) and income (P=.04) were significantly associated with the mothers’ practices regarding their children’s oral hygiene (Table 3).

A significant difference in respondents’ knowledge and practices with sociodemographic characteristics was observed (Table 4). The analysis found that the knowledge was comparatively higher among mothers of higher age groups compared to lower age groups (mean knowledge score: 12.13, 95% CI 10.73-13.54 vs 11.23, 95% CI 10.85-11.58; P=.01). Similarly, both the knowledge and practice behaviors were significantly higher among mothers with higher education and income than their counterparts. In addition, working mothers and mothers with small families had significantly higher knowledge (Table 4).

Figure 2 represents the association between mothers’ oral hygiene knowledge and practice levels. Over 50% of mothers with good knowledge had good practice behaviors regarding their children’s oral hygiene. The Pearson correlation coefficient analysis also found a significant and positive association (r=0.301; P<.001) between the knowledge and practice scores of the respondents (Multimedia Appendix 6).

Oral health is an integral component of overall health, and it is important in our everyday lives. This study intended to evaluate mothers’ knowledge and practices regarding their children’s oral hygiene. An increased knowledge level was observed among older mothers, those with higher education levels, working mothers, and mothers from higher income groups. Similarly, good practices regarding children’s oral hygiene were associated with the mother’s education level and economic status.

To maintain oral health, brushing twice a day is standard [30]. The study found that most mothers know the standard brushing recommendation for their children. Many mothers also agreed that gingival disease was the most common cause of gum bleeding, and brushing and flossing could protect against bleeding gums. The findings align with the existing literature [31]. If one wants to protect themselves against any kind of dental sickness, brushing regularly is required [32]. Over 50% of the mothers in our study agreed with this statement, which is comparable to existing research findings [33]. In this study, less than half of the mothers had good knowledge regarding their children’s oral hygiene, and nearly 1 in every 5 mothers had poor knowledge. The findings suggest that health education programs among mothers regarding their children’s oral hygiene are needed. Various education and awareness programs, including television, social and mass media campaigns, and community-based educational interventions may improve mothers’ knowledge regarding children’s oral hygiene [34-36].

In this study, the mother’s knowledge regarding their children’s oral hygiene was significantly associated with their age, and mothers in the higher age group had comparatively higher knowledge than those in the lower age group. The finding is comparable to many studies that suggest oral health educational programs for younger mothers [34,37,38]. The mother’s educational status and monthly family income were two important predictors for increasing their children’s oral hygiene knowledge and practices. Parents with higher education were more aware of their children’s dental health [39,40]. Our research results align with the existing literature that indicates that mothers who have attained a university degree possess superior knowledge about oral health in comparison to those with a lower level of education [41]. This might be rationalized by the deduction that women with a lower level of education may lack awareness about the consequences of probable risk factors linked to the progression of oral disorders. Consequently, health awareness and promotion play a vital role for mothers who have inadequate educational backgrounds [40,42,43]. Our results align with the existing research, which demonstrates that mothers with extensive knowledge tend to promote good oral health habits in their children [25].

This study aimed to identify the variables that impact oral hygiene habits among mothers and evaluate their level of knowledge and compliance with oral hygiene practices. The primary merit of this study is the results. We identified the variables that influence individuals’ understanding and behaviors related to oral hygiene. We experienced a few limitations during this study. First, this was cross-sectional research, which lacks strength in cause-effect analysis. Second, the study was conducted among mothers visiting tertiary-level hospitals in Dhaka. Therefore, there is a chance of nonresponse bias due to convenience sampling.

Maintaining good oral hygiene is crucial for every child’s overall health; mothers, in particular, play a vital role in this regard. Based on our study findings, the following recommendations may help enhance maternal knowledge and improve children’s oral hygiene practices.

This study revealed that mothers’ knowledge and practices regarding their children’s oral health were insufficient. The mother’s age, education level, family size, and monthly income significantly influenced their knowledge level. Children’s oral hygiene habits were significantly associated with family income and the mother’s educational status. Women aged 41-48 years with a bachelor’s degree or higher, from higher socioeconomic backgrounds, and with school-aged children demonstrated significantly higher levels of knowledge. Mothers with higher socioeconomic status and more education demonstrated a much higher level of dental hygiene practices for their children. The mother’s knowledge regarding their children’s oral hygiene had positive effects and significantly correlated with their children’s oral hygiene practices. The findings of this study emphasize the need for educational and school-based initiatives, accessible dental care services, oral health education in the curriculum, media and technology involvement in oral health educational campaigns, and proper research and monitoring.