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This paper [
Perhaps, explain what blastp does to provide more insight.
It would be beneficial to include a figure that displays the sequence alignment of the query sequence along with the similarity percentage.
The discussion is well-written, although could benefit from including other relevant studies/prior works to support your results.
Conclusion is relatively weak. Please consider revising it and sufficiently summarizing the Methods and Results.
Section 2.2: Please define DOPE and SOAP before abbreviating.
Discussion: It is mentioned that only 6 of 10 species could have a Km value assigned to a certain sequence, please mention these 6 species.
Discussion: The sentence “Thus it can be predicted that an enzyme with better kinetics that currently commercially available asparaginase can be cloned from Streptomyces species” is a bit ambiguous. Please rewrite this sentence.
The authors have addressed all reviewer comments and improved the manuscript. I have no further comments.
No conflict declared.